Ph.D.
Associate Professor
 
Phone: 9178000571
 

About Me

Professional summary
Over 13 years of multidisciplinary professional specializations including teaching and research experience in field of Biotechnology and Agricultural Sciences. Teaching experience in Biotechnology to post-graduate and undergraduate students with over eight years experience in screening and evaluation of germ-plasm varieties, allele mining of genes conferring salt tolerance, identification and validation of differentially expressed genes at National Institutes i.e., Indian Institute of Rice Research (IIRR)/Directorate of Rice Research (DRR), Hyderabad and National Research Centre of Plant Biotechnology (NRCPB), Indian Agricultural Research Institute (IARI), New Delhi. I possess a sound knowledge of PCR-based allele mining, qRT PCR, scanning electron microscope (SEM), drought screening techniques, physiological/biochemical analysis and microarray techniques with demonstrated ability to use statistical analysis and well versed with different Bioinformatics techniques, molecular breeding techniques and statistical analysis tools. I possess the ability to work independently as well as in a team with strong interpersonal relationship and leadership skills. 

I was an invited ‘Visiting Faculty’ under a research program on ‘Detection of freshness among cereals crops using fluorescence spectroscopy to determine the fluorescence level based on the presence of fluorescent components’ at Graduate School of Agriculture, Kyoto University, Kyoto, Japan for a period of three months from 30th November 2015 to 29th February 2016. I was felicitated with ‘Scientist Associate Award’ by Society for Scientific Development in Agriculture and Technology (SSDAT) at National Conference on Emerging Challenges and Opportunities in Biotic and Abiotic Stress Management (ECOBASM - 2014), 13th and 14th December 2014 at Directorate of Rice Research, Hyderabad. I was an ‘Invited Young Scientist with IRRI-PHILIPPINES Sponsorship’ to attend and present a part of my doctoral research work entitled ‘Whole Transcriptomic (RNA) Sequencing and Leaf Photosynthetic Response to Salinity in High Yielding Rice Introgression Lines Derived from KMR3 X O. rufipogon’, at 4th International Rice Congress (IRC2014) between 27th and 31st October 2014 at Bangkok, Thailand.
 
Research Fields

  • Plant Physiology
  • Plant Biotechnology

Teaching

  • Plant Physiology
  • Plant Biotechnology

Expertise

  • Plant DNA and RNA extraction
  • cDNA synthesis
  • polymerase chain reaction (PCR) amplification
  • validation of differentially expressed genes using qRT-PCR (ABI and Stratagen). Microarray analysis and cloning of genes tolerant to salt stress in rice introgression lines. Scanning electron microscopy (SEM) of plant samples by using QuantomiX WET-SEM capsules. Back cross breeding
  • plant tissue culture
  • micropropagation
  • callus and somatic cell induction both by leaf and stem inoculation
  • Flame photometry for ion analysis
  • Microbiology: Biochemical tests
  • Screening
  • Maintenance of pure cultures of bacteria
  • fungi and its preservation
  • Fermentation and Mushroom cultivation. Seri- Biotechnology: Silkworm rearing and breeding techniques
  • Trace element quantification by using Atomic Absorption Spectrophotometer
  • Protein quantification of membrane proteins both by Spectrophotometric and Gel electrophoretic method – Native and SDS-PAGE electrophoresis
  • DNA extraction
  • quantification and amplification from silkworm moth tissues. Computer/Bioinformatics: knowledge of bioinformatics like NCBI- pubMed
  • BLAST
  • phi BLAST
  • psi BLAST
  • FASTA
  • skilled knowledge of MS Office and soft-wares for the prediction of identification of cis-acting elements viz.
  • PLANT CARE
  • PLACE
  • JASPAR
  • etc. and skills to predict miRNA in in silico.

Interests

  • Plant Physiology
  • Plant Biotechnology

 
   

Research Fields

Screening of germ-plasm varieties, morphological, physiological, biochemical and molecular analysis like germination analysis, ion analysis using flame photometer, anti-oxidant estimations of samples at its different growth stages. Plant DNA and RNA isolation, Allele mining for salinity tolerance among introgression lines (ILs) by using SSR markers/salinity tolerant specific primers like SKC1, SHMT3, CBSDP and DREB2B was a novel study. In silico study in allele mining lead to identification of novel cis elements in salt-tolerant in comparison to salt sensitive introgression line. The whole-transcriptomic and microarray analysis for identification of differentially expressed genes in the most-tolerant and most-sensitive rice introgression lines ILs). Validated differentially expressed genes by using qRT-PCR and demonstration of expression of methionine biosynthetic genes. The cloning of SAMS1, SAMS2 and SAMS3 genes in pGEMT and pET28a expression vectors using salt-tolerant shoot samples was successfully carried out. Thus, I got exposure and experience by working in two national level, state-of-the art laboratories in the field of plant biotechnology and molecular breeding techniques under the overall guidance of two national professors both at DRR, Hyderabad and NRCPB, IARI, New Delhi.